Wisdom Jobs TOP 20 Pharma Quality Control Interview Questions and Answers 2019

[Music] question 1 what is room temperature answer 25 degree centigrade question tube what is the ultraviolet invisible spectroscopy range answer ooop spectroscopy range 200 to 400 nanometers visible spectroscopy range 400 nanometers to 800 nanometers question 3 what is the use of of spectroscopy answer spectroscopy used for detecting the functional groups impurities qualitative and quantitative analysis can be done question 4 what is the difference between qualitative and quantitative analysis answer qualitative analysis involves identification of the compound or chemical based on their chemical absorption emission or physical properties eg melting point boiling point quantitative analysis involves estimation or determination of concentration or amount of the chemical compounds or components question 5 explain the principle of ultraviolet spectroscopy answer ooop spectroscopy uses light in the O part of electromagnetic spectrum over absorption spectra arises in which molecule or atoms outer electrons absorb energy undergoes transition from lower energy level to higher energy level for each molecule absorbance at wavelength is specific question 6 explained about beer Lambert's law answer it states that the intensity of monochromatic light absorbed by a substance dissolved in a fully transmitting solvent as directly proportional to the substance concentration and the path length of the light through the solution question 7 explain the infrared spectroscopy principle answer when a molecule absorbs the infrared radiation it why braids and gives rise to pact infrared IR absorption spectrum this IR spectrum is specific for every different molecule absorbing the IR radiation useful for its identification question 8 what is the body temperature answer 37 degrees Celsius or 98.6 degrees Fahrenheit question 9 define pH what is the pH of blood answer pH neck of logarithm of hydrogen ion concentration blood pH is 7.35 to 7.45 question 10 expand lcms HPLC you PLC TLC and GC answer o LCMS liquid chromatography or HPLC high-performance liquid chromatography or you PLC ultra-high performance liquid chromatography or TLC thin layer chromatography or GC gas chromatography question 11 what is the HPLC principle answer it is a technique used for separating the mixture of components into individual components based on adsorption partition ion exchange and size exclusion principles stationary phase and mobile phase used in it HPLC used for identification quantification and purification of components form a mixture question 12 explain HPLC instrumentation answer it involves solvent system pump sample injector HPLC columns detectors and recorder firstly solvent mobile phase is degassed for eliminating the bubbles it is passed through the pump with a uniform pressure the liquid sample is injected into the mobile phase flow stream it passes through the stationary phase identified by the detectors and recorded question 13 in Reverse phase hplc which type of stationary phase is used and give example answer nonpolar stationary phase used X silica gel see 18 question 14 what are the detectors used in HPLC answer o detector IR detector fluorescence detector mass spectroscopy LCM setc question 15 how to calculate retention factor in paper chromatography answer RF equals distance traveled by solute distance traveled by solvent question 16 define molarity answer number of moles of solute per liter solution denoted with M question 17 define molality answer number of moles of solute per kilogram solvent denoted with M [Music] [Music]